作者：秦红艳，陈秀灵，赵滢，许培磊，张宝香，王月，范书田，李嘉琪，刘迎雪，李昌禹，路文鹏，艾军，杨义明

刊物名称：International Journal of Fruit Science

发表年月：2026年1月

摘要内容：The present study investigated changes in endogenous hormones and protective enzyme activities in response to Psa (biovar 2) infection in resistant and susceptible accessions of Actinidia arguta. The resistant accession “Cuiyu” and susceptible accession “TL02062” were inoculated with Psa “R12.” The activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), phenylalanine ammonia-lyase (PAL), and polyphenol oxidase (PPO), as well as contents of salicylic acid (SA), jasmonic acid (JA), total phenolic, and lignin in plant stems at days 0 (CK), 2, 7, and 14 after inoculation were assessed. After inoculation, the activities of SOD, POD, PAL, and PPO in both resistant and susceptible accessions were significantly increased. The JA content in resistant accessions increased, while that in the susceptible accessions first decreased and then increased. The total phenolic contents of resistant accessions were higher than those of susceptible accessions. The SA content was positively correlated with the JA content and with PAL and POD activities. Lignin content was negatively correlated with the levels of SA, JA, and the activities of PAL and POD. The disease resistance of A. arguta is significant correlations with the levels of SA and JA, as well as the accumulation of polyphenolic compounds. This research indicates that SA and PAL play a pivotal role in regulating the defense responses of A. arguta against Psa infection. By comparing the differential physiological responses of resistant and susceptible accessions to Psa “R12,” this study provides novel theoretical insights into the physiological and biochemical mechanisms underlying disease resistance in A. arguta, offering valuable references for guiding disease-resistant breeding programs.

Figure 1. Changes in (a) phenylalanine ammonia lyase (pal) and (b) polyphenol oxidase (PPO) activities in A. arguta stems before and after Psa inoculation. 0–14 days post inoculation (dpi) with Pseudomonas syringae pv. actinidiae (Psa) biovar 2. Enzyme activity is expressed as units (u) per gram of stem tissue. Error bars show standard error of the mean. Treatments with different lettering are statistically different, as indicated by least significant difference (LSD, p < .05). b shows that PPO activity in both “CY” and “TL02062” increased following inoculation. Prior to inoculation, resistant “CY” showed significantly lower PPO activity (21.53 U·g⁻¹) than susceptible “TL02062” (29.86 U·g⁻¹). By 2 dpi, “CY” exhibited rapid PPO activation (28.40 U·g⁻¹, 1.32-fold increase versus control) (). In contrast, “TL02062” showed no significant PPO increase until 14 dpi (p < .05).

Figure2. Changes in endogenous (a) salicylic acid (SA) and (b) jasmonic acid (JA) contents in A. arguta stems before and after Psa inoculation. 0–14 days post inoculation (dpi) with Pseudomonas syringae pv. actinidiae (Psa) biovar 2. Error bars show standard error of the mean. Treatments with different lettering are statistically different, as indicated by least significant difference (LSD, p < .05). In resistant “CY,” JA content increased significantly (p < .05) at 2, 7, and 14 dpi, peaking at 148.44 ng·g⁻¹ (2.2-fold control) by 7 dpi). Susceptible “TL02062” showed initial JA reduction (53.7% decrease at 2 dpi) followed by significant increase (p < .05) to 119.26 ng·g⁻¹ (1.27-fold control) by 14

原文链接：https://doi.org/10.1080/15538362.2025.2599875